CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS Engraftment and survival following reduced-intensity allogeneic peripheral blood hematopoietic cell transplantation is affected by CD8 T-cell dose

The influence of graft composition on clinical outcomes after reduced-intensity conditioning is not well-characterized. In this report we prospectively enumerated CD34 , CD3 , CD4 , and CD8 cell doses in granulocyte colony-stimulating factor– mobilized peripheral blood mononuclear cell (G-PBMC) allografts in 63 patients who received transplants following nonmyeloablative conditioning with total body irradiation 200 cGy plus fludarabine as treatment for malignant diseases. Donors were HLA-identical siblings (n 38) or HLA-matched unrelated individuals (n 25). By univariate analyses G-PBMC CD8 T-cell dose in at least the 50th percentile favorably correlated with full donor blood T-cell chimerism (P .03), freedom from progression (P .001), and overall survival (P .01). No G-PBMC cell dose influenced grade II to IV acute or extensive chronic graftversus-host disease. In multivariate analysis only G-PBMC CD8 T-cell dose (P .003; RR 0.2, 95% CI 0.1-0.6) was associated with improved freedom from progression. Infusion of low G-PBMC CD8 T-cell dose for reducedintensity allografting may adversely affect T-cell engraftment and survival outcome. (Blood. 2005;105:2300-2306)